Manual differential principle

Observe slide under low dry for overall impression and general appearance of blood cells. Check for even distribution of white blood cells and correct staining of cells. Observe slide under high dry lens after smearing oil droplet over length of slide.

Estimate wbc count by noting number of white cells per high power field X This number should agree with automated results. Immature granulocytes, dysplastic granulocytes, abnormal granulocytes toxic granulation, dohle bodies, hypersegmentation, pelger-huet anomaly , atypical lymphs, immature lymphs, immature monocytes, abnormal platelets, blood parasites. For manual differential count at least white blood cells under high dry lens using the LIS keyboard.

Alternatively, differential may be done under oil immersion lens. Stain quality: For slide review and manual diff answer the question in the line "Stain Quality Acceptable?

Answer "Yes" if stained cells appear according to the following:. The estimate is made by counting the average number of platelets seen per x oil immersion field in the monolayer of a well-spread smear. This value would then be compared to the reference interval for the sample in question. For the estimate, an actual count is not provided but platelets are designated into specific categories:. Increased - the platelet count is estimated to be above the reference interval.

Adequate - the platelet count is estimated to be within the reference interval. Decreased - the platelet count is estimated to be below the reference interval. Whenever possible, a red blood cell abnormality should be described in as much detail as possible. For example, when poikilocytosis is present, the type s of irregularly shaped cells should be noted; also anisocytosis, the amount of variation in the size of the red blood cells should be noted.

The generally accepted methods of reporting red blood cell irregularities include commenting on the degree of variability present Slight,moderate,marked.

Regardless of the method chosen, it should be used consistently. The reporting of red blood cell morphology varies widely between technologists. It is, therefore, helpful for each laboratory to have a uniform grading system. Also, the significance of different types of abnormal morphology will vary, and therefore, the degree of grading may depend on the abnormality present.

In addition it is important to select the proper area of the smear when determining morphology. The recommended areas on wedge smears are those fields in which some red blood cells begin to overlap. Student Health Center Manuals. Request new password. Effective Date:. Principle: The manual differential white blood cell count is performed to determine the relative number of each type of white blood cell present in the blood.

These counts are done in the same area as WBC and platelet estimates with the red cells barely touching. This takes place under X oil using the zigzag method previously described in the platelet estimate see Fig. Count WBCs including all cell lines from immature to mature. Normal values for WBCs can be found in Table If nRBCs are observed while performing the differential, they need to be reported.

These elements in a peripheral smear are indicative of increased erythropoietic activity and usually a pathologic condition. Additionally, the presence of nRBCs per white cells will falsely elevate the white count and is clinically significant. The following formula is applied for correcting NRBCs:. Grow Taller 4 Idiots. ReNew Fat Burning Supplement. Over 40 Keto Solution. The Parkinson's Disease Protocol. Rosacea Free Forever.

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Cupido What does manual diff and morphology mean? Abdullah What objective is used for bone marrow differential? Kevin How to read a bone marrow manual differential? How do you report them?